CGBE1-NG (pBM1246)

LOCUS       CGBE1-NG (pBM1246)                9909 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  CMV promoter expression plasmid for 
            UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    CGBE1-NG (pBM1246)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald
            J, Joung JK
  TITLE     CRISPR C-to-G base editors for inducing targeted DNA transversions 
            in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 
            10.1038/s41587-020-0609-x.
  PUBMED    32690971
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(46..63)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    52..276
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(347..363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(347..363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(360..382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    371..387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(395..425)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    440..461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(578..595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(749..1337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(829..848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1508..2368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2131..2150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2369..2473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2552..2571)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2743..3122
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3123..3326
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3277..3297
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3368..3387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3368..3386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3436..3456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4951..6666
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             9094..9114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9133..9189
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9190..9906
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9235..9256)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9496..9515)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9843..9864
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
       61 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      121 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      181 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
      241 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      301 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
      361 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      421 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      481 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      541 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga
     1381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     2521 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     2581 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     2641 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     2701 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     2761 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2821 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2881 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2941 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3001 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3061 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3121 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3181 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3241 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3301 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg
     3361 cggccgctaa tacgactcac tatagggaga gccgccacca tgaaacggac agccgacgga
     3421 agcgagttcg agtcaccaaa gaagaagcgg aaagtcgcca acgaactgac ctggcacgac
     3481 gtgctggccg aagagaagca gcagccctat ttccttaaca ccctgcagac cgtggccagc
     3541 gagcggcaga gcggcgtgac catctacccc ccacagaaag acgtgtttaa cgccttccgc
     3601 ttcacagagc tgggcgacgt gaaggtggtg atcctgggcc aggaccccta ccacggcccc
     3661 ggccaggccc atggtctggc cttcagcgtg cggcccggca tcgccatccc ccccagcctg
     3721 ctgaacatgt ataaggagct ggaaaacacc atccccggct tcacccggcc caatcacggc
     3781 tacctggaga gctgggcgcg gcagggcgtg ctgctgctca acaccgtgct gacggtacgg
     3841 gccggccagg cgcatagcca cgccagcctg ggctgggaga ccttcaccga taaggtgatc
     3901 agcctgatca accagcaccg ggagggcgtg gtgtttctgc tgtggggcag ccatgcccag
     3961 aagaaaggcg ccatcatcga caagcagcgg catcatgtgc tgaaagcccc ccaccccagc
     4021 ccccttagcg cccaccgggg cttcttcggc tgcaaccatt tcgtgctggc caatcagtgg
     4081 ctggaacaac ggggcgagac ccccattgac tggatgcccg tgttgcccgc cgagagcgag
     4141 agcggcggga gcggcgggag cggggggagc tcctcagaga ctgggcctgt cgccgtcgat
     4201 ccaaccctgc gccgccggat tgaacctcac gagtttgaag tgttctttga cccccgggag
     4261 ctggccaagg agacatgcct gctgtacgag atcaactggg gaggcaggca ctccatctgg
     4321 aggcacacct ctcagaacac aaataagcac gtggaggtga acttcatcga gaagtttacc
     4381 acagagcggt acttctgccc caataccaga tgtagcatca catggtttct gagctggtcc
     4441 ccttgcggag agtgtagcag ggccatcacc gagttcctgt ccagatatcc acacgtgaca
     4501 ctgtttatct acatcgccag gctgtatcac cacgcagacc caaggaatag gcagggcctg
     4561 cgcgatctga tcagctccgg cgtgaccatc cagatcatga cagagcagga gtccggctac
     4621 tgctggcgga acttcgtgaa ttattctcct agcaacgagg cccactggcc taggtaccca
     4681 cacctgtggg tgcgcctgta cgtgctggag ctgtattgca tcatcctggg cctgccccct
     4741 tgtctgaata tcctgcggag aaagcagccc cagctgacct tctttacaat cgccctgcag
     4801 tcttgtcact atcagaggct gccaccccac atcctgtggg ccacaggcct gaagtctgga
     4861 ggatctagcg gaggatcctc tggcagcgag acaccaggaa caagcgagtc agcaacacca
     4921 gagagcagtg gcggcagcag cggcggcagc gacaagaagt acagcatcgg cctggccatc
     4981 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
     5041 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg
     5101 ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga
     5161 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc
     5221 aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag
     5281 aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
     5341 taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg
     5401 cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag
     5461 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc
     5521 tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc
     5581 ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc
     5641 gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac
     5701 ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac
     5761 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg
     5821 gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag
     5881 atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac
     5941 ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc
     6001 ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag
     6061 ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg
     6121 aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc
     6181 caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca
     6241 ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac
     6301 gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa
     6361 accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc
     6421 atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac
     6481 agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc
     6541 gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg
     6601 ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa
     6661 atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg
     6721 ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa
     6781 aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg
     6841 atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg
     6901 aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg
     6961 gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga
     7021 aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc
     7081 caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc
     7141 gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg
     7201 ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag
     7261 aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg
     7321 ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg
     7381 tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac
     7441 cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc
     7501 atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc
     7561 tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg
     7621 attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg
     7681 gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg
     7741 gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg
     7801 gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag
     7861 ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
     7921 gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc
     7981 gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag
     8041 gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc
     8101 ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag
     8161 atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa
     8221 gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatccgg
     8281 cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac
     8341 ggcggcttcg tgagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag
     8401 ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga
     8461 agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa
     8521 aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag
     8581 agaatgctgg cctctgcccg gttcctgcag aagggaaacg aactggccct gccctccaaa
     8641 tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat
     8701 aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag
     8761 cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg
     8821 tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac
     8881 ctgtttaccc tgaccaatct gggagcccct cgggccttca agtactttga caccaccatc
     8941 gaccggaagg tgtaccggag caccaaagag gtgctggacg ccaccctgat ccaccagagc
     9001 atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctctggcggc
     9061 tcaaaaagaa ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtcggaggc
     9121 ggtggaagcg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat
     9181 cctggaccga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     9241 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     9301 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     9361 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     9421 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     9481 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     9541 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     9601 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     9661 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     9721 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     9781 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     9841 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     9901 tacaagtga
//